2025 Columbus Challenger

Country	Player	Rank1	Seed
ARG	Juan Pablo Ficovich	132	1
USA	Murphy Cassone	177	2
JPN	James Trotter	187	3
COL	Nicolás Mejía	200	4
GBR	Johannus Monday	238	5
USA	Andres Martin	270	6
USA	Martin Damm	281	7
USA	Garrett Johns	293	8

• ¹ Rankings are as of September 8, 2025.^[1]

The following players received entry into the singles main draw as wildcards:

• Jack Anthrop
• Alexander Bernard
• Aidan Kim

The following player received entry into the singles main draw using a protected ranking:

• Philip Sekulic

The following player received entry into the singles main draw through the Junior Accelerator programme:

• Théo Papamalamis

The following players received entry into the singles main draw as alternates;

• Petr Brunclík
• Martin Damm

The following players received entry from the qualifying draw:

• Samir Banerjee
• Justin Boulais
• Sebastian Dominko
• Antoine Ghibaudo
• Daniel Masur
• Preston Stearns

• Michael Zheng def. Martin Damm 3–6, 6–3, 7–5.

• Patrick Harper / Johannus Monday def. George Goldhoff / Theodore Winegar 6–4, 6–3.