2025 Knoxville Challenger

Country	Player	Rank1	Seed
JPN	James Trotter	198	1
GBR	Jay Clarke	203	2
COL	Nicolás Mejía	205	3
GBR	Johannus Monday	212	4
USA	Mitchell Krueger	242	5
EST	Daniil Glinka	263	6
USA	Alex Rybakov	264	7
USA	Andres Martin	270	8

• ¹ Rankings are as of October 27, 2025.^[1]

The following players received wildcards into the singles main draw:

• Andrew Fenty
• Alexander Kotzen
• Dominique Rolland

The following player received entry into the singles main draw through the Junior Accelerator programme:

• Roh Ho-young

The following player received entry into the singles main draw as an alternate:

• Stefan Kozlov

The following players received entry from the qualifying draw:

• Murphy Cassone
• Péter Makk
• Shunsuke Mitsui
• Francisco Rocha
• Dhakshineswar Suresh
• Max Wiskandt

The following player received entry as a lucky loser:

• Leo Borg

• Mitchell Krueger def. Darwin Blanch 6–7^(2–7), 6–4, 6–1.

• Patrick Harper / Quinn Vandecasteele def. Mitchell Krueger / Jody Maginley 6–7^(6–8), 7–6^(7–4), [12–10].