2021 Amex-Istanbul Challenger

Country	Player	Rank1	Seed
COL	Daniel Elahi Galán	115	1
BRA	Thiago Seyboth Wild	117	2
FRA	Antoine Hoang	121	3
ARG	Facundo Bagnis	125	4
IND	Prajnesh Gunneswaran	128	5
ITA	Lorenzo Musetti	129	6
SVK	Jozef Kovalík	131	7
JPN	Go Soeda	134	8

• ¹ Rankings are as of 11 January 2021.^[1]

The following players received wildcards into the singles main draw:

• Altuğ Çelikbilek
• Cem İlkel
• Ergi Kırkın

The following players received entry from the qualifying draw:

• Jay Clarke
• João Menezes
• Arthur Rinderknech
• Lukáš Rosol

The following player received entry as a lucky loser:

• Denis Istomin

• Arthur Rinderknech def. Benjamin Bonzi 4–6, 7–6^(7–1), 7–6^(7–3).

• André Göransson / David Pel def. Lloyd Glasspool / Harri Heliövaara 4–6, 6–3, [10–8].