2021 Internazionali di Tennis Città di Todi

Country	Player	Rank1	Seed
ITA	Federico Gaio	145	1
ESP	Mario Vilella Martínez	179	2
KAZ	Dmitry Popko	187	3
ARG	Tomás Martín Etcheverry	192	4
ARG	Renzo Olivo	193	5
ARG	Andrea Collarini	206	6
ITA	Gian Marco Moroni	218	7
ITA	Lorenzo Giustino	228	8

• ¹ Rankings are as of 28 June 2021.^[2]

The following players received wildcards into the singles main draw:

• Flavio Cobolli
• Francesco Forti
• Francesco Passaro

The following player received entry into the singles main draw using a protected ranking:

• Viktor Galović

The following players received entry into the singles main draw as alternates:

• Facundo Díaz Acosta
• Skander Mansouri

The following players received entry from the qualifying draw:

• Matteo Arnaldi
• Arthur Cazaux
• Giovanni Fonio
• Matheus Pucinelli de Almeida

The following player received entry as a lucky loser:

• Franco Agamenone

• Mario Vilella Martínez def. Federico Gaio 7–6^(7–3), 1–6, 6–3.

• Francesco Forti / Giulio Zeppieri def. Facundo Díaz Acosta / Alexander Merino 6–3, 6–2.