2021 Città di Como Challenger

Country	Player	Rank1	Seed
GER	Daniel Altmaier	106	1
SVK	Jozef Kovalík	121	2
SVK	Andrej Martin	122	3
ARG	Juan Manuel Cerúndolo	135	4
ITA	Federico Gaio	159	5
IND	Sumit Nagal	165	6
SUI	Marc-Andrea Hüsler	170	7
ITA	Alessandro Giannessi	171	8

• ¹ Rankings are as of 23 August 2021.^[1]

The following players received wildcards into the singles main draw:

• Flavio Cobolli
• Francesco Forti
• Federico Iannaccone

The following players received entry into the singles main draw using protected rankings:

• Andrea Arnaboldi
• Julien Cagnina
• Julian Lenz

The following players received entry into the singles main draw as special exempts:

• Nino Serdarušić
• Camilo Ugo Carabelli

The following players received entry into the singles main draw as alternates:

• Evan Furness
• Giulio Zeppieri

The following players received entry from the qualifying draw:

• Petros Chrysochos
• Alexander Erler
• Lucas Miedler
• Andrea Vavassori

The following players received entry as lucky losers:

• Franco Agamenone
• Alexey Vatutin

• Juan Manuel Cerúndolo def. Gian Marco Moroni 7–5, 7–6^(9–7).

• Rafael Matos / Felipe Meligeni Alves def. Luis David Martínez / Andrea Vavassori 6–7^(2–7), 6–4, [10–6].