2021 Challenger Concepción

Country	Player	Rank1	Seed
ARG	Federico Coria	92	1
SVK	Andrej Martin	103	2
POR	Pedro Sousa	107	3
BOL	Hugo Dellien	112	4
COL	Daniel Elahi Galán	115	5
BRA	Thiago Seyboth Wild	117	6
ARG	Facundo Bagnis	127	7
GER	Daniel Altmaier	134	8

• ¹ Rankings as of 8 February 2021.^[1]

The following players received wildcards into the singles main draw:

• Nicolás Álvarez
• Nicolás Jarry
• Gonzalo Lama

The following player received entry into the singles main draw as an alternate:

• Camilo Ugo Carabelli

The following players received entry from the qualifying draw:

• Hernán Casanova
• Carlos Gómez-Herrera
• Vitaliy Sachko
• Thiago Agustín Tirante

• Sebastián Báez def. Francisco Cerúndolo 6–3, 6–7^(5–7), 7–6^(7–5).

• Orlando Luz / Rafael Matos def. Sergio Galdós / Diego Hidalgo 7–5, 6–4.