2021 Florianópolis Challenger

Country	Player	Rank1	Seed
URU	Pablo Cuevas	99	1
BOL	Hugo Dellien	119	2
BRA	Thiago Seyboth Wild	131	3
ARG	Juan Ignacio Londero	139	4
ARG	Nicolás Kicker	229	5
ARG	Pedro Cachin	239	6
ARG	Andrea Collarini	264	7
ARG	Juan Pablo Ficovich	285	8

• ¹ Rankings are as of 29 November 2021.^[1]

The following players received wildcards into the singles main draw:

• Mateus Alves
• Pedro Boscardin Dias
• Gustavo Heide

The following players received entry into the singles main draw as alternates:

• Luciano Darderi
• Gonzalo Villanueva
• Matías Zukas

The following players received entry from the qualifying draw:

• Nicolás Barrientos
• Wilson Leite
• Igor Marcondes
• Federico Zeballos

• Igor Marcondes def. Hugo Dellien 6–2, 6–4.

• Nicolás Barrientos / Alejandro Gómez def. Martín Cuevas / Rafael Matos 6–3, 6–3.