2021 Quito Challenger

Country	Player	Rank1	Seed
SVK	Andrej Martin	122	1
ECU	Emilio Gómez	161	2
ESP	Mario Vilella Martínez	170	3
ARG	Renzo Olivo	215	4
ARG	Juan Pablo Ficovich	254	5
ARG	Facundo Mena	262	6
ARG	Thiago Agustín Tirante	268	7
ECU	Roberto Quiroz	294	8

• ¹ Rankings are as of 30 August 2021.^[1]

The following players received wildcards into the singles main draw:

• Álvaro Guillén Meza
• Antonio Cayetano March
• Gian Carlos Rodríguez

The following players received entry from the qualifying draw:

• Luca Castelnuovo
• Alexis Gautier
• Alejandro Gómez
• Christian Langmo

• Facundo Mena def. Gonzalo Lama 6–4, 6–4.

• Alejandro Gómez / Thiago Agustín Tirante def. Adrián Menéndez Maceiras / Mario Vilella Martínez 7–5, 6–7^(5–7), [10–8].