2021 Garden Open II

Country	Player	Rank1	Seed
BIH	Damir Džumhur	116	1
BOL	Hugo Dellien	125	2
ITA	Federico Gaio	138	3
SRB	Danilo Petrović	157	4
FRA	Hugo Gaston	158	5
ITA	Paolo Lorenzi	159	6
PER	Juan Pablo Varillas	160	7
ITA	Alessandro Giannessi	165	8

• ¹ Rankings as of 19 April 2021.^[1]

The following players received wildcards into the singles main draw:

• Flavio Cobolli
• Stefano Napolitano
• Giulio Zeppieri

The following player received entry into the singles main draw as a special exempt:

• Andrea Pellegrino

The following player received entry into the singles main draw using a protected ranking:

• Thanasi Kokkinakis

The following player received entry into the singles main draw as an alternate:

• Andrea Collarini

The following players received entry from the qualifying draw:

• Tristan Lamasine
• Felipe Meligeni Alves
• Nino Serdarušić
• Igor Sijsling

• Juan Manuel Cerúndolo def. Flavio Cobolli 6–2, 3–6, 6–3.

• Sadio Doumbia / Fabien Reboul def. Guido Andreozzi / Guillermo Durán 7–5, 6–3.