2022 Ambato La Gran Ciudad

Country	Player	Rank1	Seed
PER	Juan Pablo Varillas	104	1
ARG	Facundo Bagnis	112	2
ARG	Juan Pablo Ficovich	144	3
ARG	Facundo Mena	159	4
ARG	Santiago Rodríguez Taverna	169	5
AUT	Gerald Melzer	222	6
SRB	Miljan Zekić	242	7
ARG	Thiago Agustín Tirante	243	8

• ¹ Rankings are as of 10 October 2022.^[1]

The following players received wildcards into the singles main draw:

• Andrés Andrade
• Álvaro Guillén Meza
• Cayetano March

The following player received entry into the singles main draw using a protected ranking:

• Jan Choinski

The following players received entry into the singles main draw as alternates:

• Sean Cuenin
• Arklon Huertas del Pino

The following players received entry from the qualifying draw:

• Sriram Balaji
• Conner Huertas del Pino
• Kosuke Ogura
• Kiranpal Pannu
• Jorge Panta
• Matías Zukas

• Facundo Bagnis def. João Lucas Reis da Silva 7–6^(9–7), 6–4.

• Santiago Rodríguez Taverna / Thiago Agustín Tirante def. Benjamin Lock / Courtney John Lock 7–6^(13–11), 6–3.