2022 Challenger del Biobío

Country	Player	Rank1	Seed
BOL	Hugo Dellien	101	1
PER	Juan Pablo Varillas	117	2
ARG	Tomás Martín Etcheverry	123	3
CHI	Nicolás Jarry	142	4
CHI	Tomás Barrios Vera	145	5
CZE	Vít Kopřiva	165	6
ARG	Camilo Ugo Carabelli	175	7
ARG	Facundo Mena	183	8

• ¹ Rankings are as of 7 March 2022.^[1]

The following players received wildcards into the singles main draw:

• Gonzalo Bueno
• Diego Fernández Flores
• Daniel Antonio Núñez

The following players received entry into the singles main draw as alternates:

• Evan Furness
• Paul Jubb
• Genaro Alberto Olivieri

The following players received entry from the qualifying draw:

• Hernán Casanova
• Corentin Denolly
• Daniel Dutra da Silva
• Facundo Juárez
• Juan Bautista Torres
• Gonzalo Villanueva

• Tomás Martín Etcheverry def. Hugo Dellien 6–3, 6–2.

• Andrea Collarini / Renzo Olivo def. Diego Hidalgo / Cristian Rodríguez 6–4, 6–4.