2022 Challenger Ciudad de Guayaquil

Country	Player	Rank1	Seed
ARG	Federico Coria	73	1
ARG	Tomás Martín Etcheverry	89	2
GER	Daniel Altmaier	106	3
PER	Juan Pablo Varillas	121	4
ARG	Juan Manuel Cerúndolo	136	5
ITA	Franco Agamenone	159	6
ARG	Santiago Rodríguez Taverna	162	7
BRA	Felipe Meligeni Alves	169	8

• ¹ Rankings are as of 24 October 2022.^[1]

The following players received wildcards into the singles main draw:

• Andrés Andrade
• Álvaro Guillén Meza
• Cayetano March

The following player received entry into the singles main draw using a protected ranking:

• Sumit Nagal

The following players received entry into the singles main draw as alternates:

• Nikola Milojević
• Roberto Quiroz
• Nikolás Sánchez Izquierdo

The following players received entry from the qualifying draw:

• Blaise Bicknell
• Marco Cecchinato
• Jan Choinski
• Juan Ignacio Galarza
• Eduardo Ribeiro
• Thiago Seyboth Wild

The following player received entry as a lucky loser:

• Román Andrés Burruchaga

• Daniel Altmaier def. Federico Coria 6–2, 6–4.

• Guido Andreozzi / Guillermo Durán def. Facundo Díaz Acosta / Luis David Martínez 6–0, 6–4.